TGBIBAC
| Library Name: | TG BIBAC Library |
|---|---|
| Library type: | BIBAC |
| Accession: | Musa acuminata Tuu Gia |
| Plant material: | Youngest fully developed leaves |
| Vector: | pCLD04541 |
| Host strain: | E.coli DH 10B |
| Restriction site: | HindIII |
| Insert size (kbp): | 100 |
| Ligation: | 1 |
| Coverage: | 5.1 |
| Number of clones: | 30700 |
| Number of plates: | 80 |
| Number/type of filters: | 2 (Gridding pattern 4x4) 4 (Gridding pattern 3x3) |
| Constructor(s): | James A. Ortiz-Vazquez E. |
| date of birth (YYYY-DD-MM): | 2003-01-08 |
| Place of birth: | CICY |
| specifications: | Live bacterial clones in 384-well plates |
| Curator(s): | James A. Seifertova M. |
| Storage place(s): | CICY IEB |
| Contruction method: | Nuclei were purified by centrifugation and embedded in agarose plugs. Megabase DNA was partially digested by HindIII, subjected to two rounds of size selection by PFGE, electro- eluted and ligated to the linearized and dephosphorylated vector. |