MBP
| Library Name: | PKW BAC Library |
|---|---|
| Library type: | BAC |
| Accession: | Musa balbisiana Pisang Klutuk Wulung |
| Plant material: | Greenhouse-grown leaves |
| Vector: | pIndigoBAC-5 |
| Host strain: | E.coli DH 10B |
| Restriction site: | HindIII |
| Insert size (kbp): | 135 |
| Ligation: | 3 |
| Coverage: | 9 |
| Number of clones: | 36864 |
| Number of plates: | 96 |
| Number/type of filters: | 2 (Gridding pattern 4x4) 3 (Gridding pattern 3x3) |
| Constructor(s): | Piffannlli P. Safar J. |
| date of birth (YYYY-DD-MM): | 2002-01-03 |
| Place of birth: | CIRAD |
| specifications: | Live bacterial clones in 384-well plates |
| Curator(s): | Seifertova M. |
| Storage place(s): | CIRAD IEB |
| Contruction method: | Nuclei were purified by centrifugation and/or flow cytometry and embedded in agarose plugs. Megabase DNA was partially digested with HindIII, subjected to two rounds of size selection by PFGE, electro- eluted and ligated to the linearized and dephosphorylated vector. |