C4BAM
| Library Name: | C4BAM BAC Library |
|---|---|
| Library type: | BAC |
| Accession: | Musa acuminata Calcutta 4 |
| Plant material: | Field-grown cigar leaves |
| Vector: | pECBAC-1 |
| Host strain: | E.coli DH 10B |
| Restriction site: | BamHI |
| Insert size (kbp): | 110 |
| Ligation: | 1 |
| Coverage: | 3 |
| Number of clones: | 17280 |
| Number of plates: | 45 |
| Number/type of filters: | 1 (Gridding pattern 4x4) 2 (Gridding pattern 3x3) |
| Constructor(s): | James A. Tao Q. |
| date of birth (YYYY-DD-MM): | 1999-01-03 |
| Place of birth: | A&M |
| specifications: | Live bacterial clones in 384-well plates |
| Curator(s): | Seifertova M. Zhang H. |
| Storage place(s): | A&M IEB |
| Contruction method: | Nuclei were purified by centrifugation and embedded in agarose plugs. Megabase DNA was partially digested with Bam |